Jessica A. Zapata-García, School of Medicine, Deanship of Health Sciences, Universidad Autónoma de Guadalajara, Zapopan, Jal., Mexico
Background: Leukemic stem cells (LSC) are characterized by excessive proliferation, self-renewal capacity, and chemoresistance, these attributes contribute to the complexity of leukemia treatment. Objective: To identify differences in the transcriptome of two LSC profiles. Methods: RNA sequencing analysis was conducted on CD99 high and t(8;21) LSC profiles, with the results subsequently correlated with the overall survival of patients with myeloid leukemia. Results: The CD99 high profile was found to be regulated by senescence mechanisms, RNA modulation, WNT, RHO GTPASES, and interleukin 7 signaling pathways, whereas the t(8;21) cells by small molecule transport, RNA polymerase II, and G protein alpha and GPCR-mediated signaling pathways. Furthermore, high expression of 19 genes of the t(8;21) profile and 4 of the high CD99 profile correlated with poor survival in patients with myeloid leukemia treated with chemotherapy. Conclusion: This study provides novel information leading to the conclusion that the LSC profiles analyzed in this work exhibit divergent transcriptomic regulation that may benefit chemoresistant capacity in the context of myeloid leukemia.
Keywords: Células madre leucémicas. CD99. t(8;21). Quimiorresistencia. Pronóstico.
